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recoverall extraction kit #am1975  (Thermo Fisher)


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    Structured Review

    Thermo Fisher recoverall extraction kit #am1975
    Recoverall Extraction Kit #Am1975, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recoverall+extraction+kit/recoverall+total+nucleic+acid+isolation+kit+for+ffpe/pmc11677537-140-1-5
    Average 90 stars, based on 1 article reviews
    recoverall extraction kit #am1975 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Utilisation of semiconductor sequencing for the detection of predictive biomarkers in glioblastoma
    Article Snippet: DNA and RNA was extracted from PWET curls cut at 10μm or from 5μm sections mounted onto unstained glass slides using the RecoverAllTM extraction kit (Ambion, Cat: A26069).

    Extraction:

    Article Title: MicroRNA miR-J1-5p as a potential Biomarker for JC Virus Infection in the Gastrointestinal Tract
    Article Snippet: Total RNA, including miRNAs, was isolated from frozen cell pellets, frozen tissues and stool samples using the miRNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions with some modifications for stool samples as previously described . .. RecoverAll extraction kit (Life Technologies, Inc., Carlsbad, CA) was used to isolate total RNA from 5-μm formalin-fixed, paraffin-embedded (FFPE) sections according to the manufacturer’s instructions . ..

    Article Title: Molecular characterization of non-small cell lung cancer tumors in Latin American patients from Brazil, Chile and Peru uncovers novel potentially driver mutations
    Article Snippet: .. Up to 8 FFPE 5 μm sections with at least 5% of tumor cellularity were selected for isolation of RNA and DNA, using the RecoverAll extraction kit (Thermo Fisher Scientific). .. Libraries were prepared using Oncomine Focus Assay (OFA, Thermo Fisher Scientific) and sequenced in the Ion Personal Genome Machine System.

    Article Title: Utilisation of semiconductor sequencing for detection of actionable fusions in solid tumours
    Article Snippet: .. RNA was extracted from FFPE curls cut at 10μm or from 5μm sections mounted onto unstained glass slides using the RecoverAll extraction kit (Ambion, Cat:A26069). ..

    Article Title: MET Exon 14 Skipping and Novel Actionable Variants: Diagnostic and Therapeutic Implications in Latin American Non-Small-Cell Lung Cancer Patients
    Article Snippet: .. The RecoverAll extraction kit (#AM1975 Thermo Fisher Scientific, Carlsland, CA, USA) was used to isolate RNA and DNA. .. The Oncomine Focus Assay (OFA, Thermo Fisher Scientific, Carlsland, CA, USA) was used to prepare the libraries, and they were sequenced in the Ion Personal Genome Machine System to perform NGS.

    Article Title: The evolutionarily conserved long non‐coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms
    Article Snippet: .. RNA was isolated from the FFPE blocks using the RecoverAll Extraction Kit (Thermo). .. For each sample, an average of 800 ng of total RNA was reverse‐transcribed using the SuperScript VILO cDNA Synthesis Kit (Thermo).

    Article Title: Utilisation of semiconductor sequencing for detection of actionable fusions in solid tumours
    Article Snippet: .. RNA was extracted from FFPE curls cut at 10μm or from 5μm sections mounted onto unstained glass slides using the RecoverAll extraction kit (Ambion, Cat no.A26069). ..

    Article Title: N-cadherin and FGFR1 and/or FGFR4 for use in predicting the response of patients to a lung cancer treatment and method and kit based on said use
    Article Snippet: .. Total RNA was extracted using the RecoverAll extraction kit (Life Technologies), following the manufacturers instructions for the extraction of RNA from biological tissue. .. Once the RNA was extracted, its concentration was quantified using the NanoDrop equipment (ThermoScientific) and stored at −80° C. for later use.

    Formalin-fixed Paraffin-Embedded:

    Article Title: MicroRNA miR-J1-5p as a potential Biomarker for JC Virus Infection in the Gastrointestinal Tract
    Article Snippet: Total RNA, including miRNAs, was isolated from frozen cell pellets, frozen tissues and stool samples using the miRNeasy Mini kit (QIAGEN) according to the manufacturer’s instructions with some modifications for stool samples as previously described . .. RecoverAll extraction kit (Life Technologies, Inc., Carlsbad, CA) was used to isolate total RNA from 5-μm formalin-fixed, paraffin-embedded (FFPE) sections according to the manufacturer’s instructions . ..

    Article Title: Molecular characterization of non-small cell lung cancer tumors in Latin American patients from Brazil, Chile and Peru uncovers novel potentially driver mutations
    Article Snippet: .. Up to 8 FFPE 5 μm sections with at least 5% of tumor cellularity were selected for isolation of RNA and DNA, using the RecoverAll extraction kit (Thermo Fisher Scientific). .. Libraries were prepared using Oncomine Focus Assay (OFA, Thermo Fisher Scientific) and sequenced in the Ion Personal Genome Machine System.

    Article Title: Utilisation of semiconductor sequencing for detection of actionable fusions in solid tumours
    Article Snippet: .. RNA was extracted from FFPE curls cut at 10μm or from 5μm sections mounted onto unstained glass slides using the RecoverAll extraction kit (Ambion, Cat:A26069). ..

    Article Title: The evolutionarily conserved long non‐coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms
    Article Snippet: .. RNA was isolated from the FFPE blocks using the RecoverAll Extraction Kit (Thermo). .. For each sample, an average of 800 ng of total RNA was reverse‐transcribed using the SuperScript VILO cDNA Synthesis Kit (Thermo).

    Article Title: Utilisation of semiconductor sequencing for detection of actionable fusions in solid tumours
    Article Snippet: .. RNA was extracted from FFPE curls cut at 10μm or from 5μm sections mounted onto unstained glass slides using the RecoverAll extraction kit (Ambion, Cat no.A26069). ..

    Isolation:

    Article Title: Molecular characterization of non-small cell lung cancer tumors in Latin American patients from Brazil, Chile and Peru uncovers novel potentially driver mutations
    Article Snippet: .. Up to 8 FFPE 5 μm sections with at least 5% of tumor cellularity were selected for isolation of RNA and DNA, using the RecoverAll extraction kit (Thermo Fisher Scientific). .. Libraries were prepared using Oncomine Focus Assay (OFA, Thermo Fisher Scientific) and sequenced in the Ion Personal Genome Machine System.

    Article Title: The evolutionarily conserved long non‐coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms
    Article Snippet: .. RNA was isolated from the FFPE blocks using the RecoverAll Extraction Kit (Thermo). .. For each sample, an average of 800 ng of total RNA was reverse‐transcribed using the SuperScript VILO cDNA Synthesis Kit (Thermo).



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    Image Search Results


    The RNA and DNA MET sequencing analysis evidenced differences in diagnosing the MET exon 14 skipping variant. ( A ) DNA regions of exons 13, 14, 15, and introns 14 and 15 of the MET gene (GRCh37.p13). Below are the DNA variants’ locations, which affect the coding sequence of exon 14 and the splicing donor region of the MET gene (variants located in red in rows) of each patient with a DNA variant in the SD region. ( B ) The broad spectrum of the RNA reads for the METex14 variant is shown in the x-axis. Each column represents a patient; the dashedline shows the threshold (120 reads) for the positive METex14 diagnosis . ( C ) The number of patients categorized as negative and positive for METex14, according to the numbers of RNA reads. ( D ) The conceptual map represents all TBx from the NSCLC patients with a pair of RNA- and DNA-sequenced QC pass data. ( E ) The Pearson correlation between the RNA and DNA reads is represented by a continous line and the standard error as a shadow. ( F ) The Pearson correlation analysis between the allele frequency of the positive METex14 DNA variants (X-axis) and the number of RNA reads (Y-axis). ( G ) Altered genes in the tumor profile of the patients with low RNA reads for METex14 .

    Journal: International Journal of Molecular Sciences

    Article Title: MET Exon 14 Skipping and Novel Actionable Variants: Diagnostic and Therapeutic Implications in Latin American Non-Small-Cell Lung Cancer Patients

    doi: 10.3390/ijms252413715

    Figure Lengend Snippet: The RNA and DNA MET sequencing analysis evidenced differences in diagnosing the MET exon 14 skipping variant. ( A ) DNA regions of exons 13, 14, 15, and introns 14 and 15 of the MET gene (GRCh37.p13). Below are the DNA variants’ locations, which affect the coding sequence of exon 14 and the splicing donor region of the MET gene (variants located in red in rows) of each patient with a DNA variant in the SD region. ( B ) The broad spectrum of the RNA reads for the METex14 variant is shown in the x-axis. Each column represents a patient; the dashedline shows the threshold (120 reads) for the positive METex14 diagnosis . ( C ) The number of patients categorized as negative and positive for METex14, according to the numbers of RNA reads. ( D ) The conceptual map represents all TBx from the NSCLC patients with a pair of RNA- and DNA-sequenced QC pass data. ( E ) The Pearson correlation between the RNA and DNA reads is represented by a continous line and the standard error as a shadow. ( F ) The Pearson correlation analysis between the allele frequency of the positive METex14 DNA variants (X-axis) and the number of RNA reads (Y-axis). ( G ) Altered genes in the tumor profile of the patients with low RNA reads for METex14 .

    Article Snippet: The RecoverAll extraction kit (#AM1975 Thermo Fisher Scientific, Carlsland, CA, USA) was used to isolate RNA and DNA.

    Techniques: Sequencing, Variant Assay